Mapping mutations to the SARS-CoV-2 RBD that escape binding by different classes of antibodies

Abstract Monoclonal antibodies targeting a variety of epitopes have been isolated from individuals previously infected with SARS-CoV-2, but the relative contributions these different antibody classes to polyclonal response remains unclear. Here we use yeast-display system map all mutations viral spike receptor-binding domain (RBD) that escape binding by representatives three potently neutralizing anti-RBD high-resolution structures. We compare antibody-escape maps similar for convalescent plasmas, including plasmas whom some were isolated. While plasma are affected across multiple RBD epitopes, plasma-escape most resemble those single class target an epitope on includes site E484. Therefore, although human immune can produce diverse in practice infection is skewed towards already undergoing rapid evolution.